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Kenjiro W. Quides, Glenna M. Stomackin, Hsu-Han Lee, Jeff H. Chang, Joel L. Sachs
Lotus japonicus alters in planta fitness of Mesorhizobium loti dependent on symbiotic nitrogen fixation
www.plosone.org
www.plosone.org
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(without)Tj
3.3278 0 Td
(any)Tj
1.6611 0 Td
(other)Tj
2.3471 0 Td
(source)Tj
2.8346 0 Td
(of)Tj
1.0148 0 Td
(nitrogen)Tj
-32.3827 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(56)Tj
0 g
(].)Tj
/F8 1 Tf
2.0749 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9751 0 Td
[()3()12()4(\n)11()18()4()1()-4( )]TJ
/F7 1 Tf
3.9231 0 Td
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2.5682 0 Td
(determinate)Tj
5.057 0 Td
(nodules)Tj
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(lack)Tj
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(a)Tj
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(continuous)Tj
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(meristem.)Tj
/F8 1 Tf
4.2349 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9695 0 Td
[()3()12()4(\n)11()18()4()1()-4( )]TJ
/F7 1 Tf
-32.1503 -1.2982 Td
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3.3618 0 Td
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(intracellular)Tj
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(rhizobia)Tj
3.5092 0 Td
(\(i.e.,)Tj
-35.1096 -1.2983 Td
(bacteroids\),)Tj
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(cease)Tj
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(growth)Tj
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(complete,)Tj
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(and)Tj
1.7348 0 Td
(because)Tj
3.3165 0 Td
(the)Tj
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(bacteroids)Tj
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(do)Tj
-36.5213 -1.3039 Td
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4.3313 0 Td
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5.3065 0 Td
(allow)Tj
2.3357 0 Td
(rhizobia)Tj
3.5149 0 Td
(to)Tj
1.0262 0 Td
(escape)Tj
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(back)Tj
2.0806 0 Td
(into)Tj
1.8425 0 Td
(the)Tj
1.4683 0 Td
(soil)Tj
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(nodule)Tj
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(senes-)Tj
-33.8057 -1.2983 Td
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2.6872 0 Td
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1.9842 0 Td
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(contrast)Tj
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(other)Tj
2.3471 0 Td
(legumes)Tj
3.4866 0 Td
(such)Tj
2.0636 0 Td
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/F8 1 Tf
1.0148 0 Td
[()28()7()12()4()7()15()2(\n)]TJ
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[()7()-9()12()6()7()22()7()4()4()]TJ
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4.3427 0 Td
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(indetermi-)Tj
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1.7801 0 Td
(grow)Tj
2.2451 0 Td
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4.7791 0 Td
(the)Tj
1.4684 0 Td
(functional)Tj
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(association,)Tj
4.8586 0 Td
(and)Tj
1.7291 0 Td
(have)Tj
2.0579 0 Td
(a)Tj
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(spatial)Tj
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(gradation)Tj
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(of)Tj
-36.0167 -1.2982 Td
(bacteroids)Tj
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1.0375 0 Td
(different)Tj
3.6567 0 Td
(developmental)Tj
6.0887 0 Td
(stages.)Tj
2.7893 0 Td
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1.7802 0 Td
(bacteroids)Tj
4.3426 0 Td
(of)Tj
1.0148 0 Td
(indeterminate)Tj
5.8734 0 Td
(nodules)Tj
3.3618 0 Td
(tend)Tj
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-36.3002 -1.3039 Td
(terminally)Tj
4.3312 0 Td
(differentiate)Tj
5.074 0 Td
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1.7348 0 Td
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(the)Tj
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(nodule,)Tj
3.2258 0 Td
(but)Tj
1.5534 0 Td
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-34.9792 -1.2983 Td
(bia)Tj
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1.6214 0 Td
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3.4355 0 Td
(upon)Tj
2.3187 0 Td
(nodule)Tj
2.9991 0 Td
(senescence)Tj
4.5807 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(57)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(58)Tj
0 g
(].)Tj
-16.3387 -1.2983 Td
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1.1111 0 Td
(the)Tj
/F8 1 Tf
1.4684 0 Td
[()-3(\n)12()7()-4( )]TJ
/F7 1 Tf
(-)Tj
/F8 1 Tf
[()28()8( )-3(\n)11()8()18(\r)-3(\n)13()11()12()12()]TJ
/F7 1 Tf
8.8383 0 Td
(symbiosis)Tj
4.1272 0 Td
(both)Tj
2.058 0 Td
(symbiotic)Tj
4.1272 0 Td
(partners)Tj
3.5433 0 Td
(have)Tj
2.0636 0 Td
(been)Tj
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(developed)Tj
4.2746 0 Td
(as)Tj
-34.9282 -1.3039 Td
(models)Tj
3.101 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(molecular)Tj
4.218 0 Td
(and)Tj
1.7347 0 Td
(cellular)Tj
3.1351 0 Td
(basis)Tj
2.1487 0 Td
(of)Tj
1.0148 0 Td
(nodulation)Tj
4.6374 0 Td
([)Tj
0.83 0.64 0.02 0 k
(59)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(60)Tj
0 g
(].)Tj
3.2485 0 Td
(Mutants)Tj
3.583 0 Td
(of)Tj
1.0148 0 Td
(MAFF)Tj
2.8516 0 Td
(have)Tj
2.058 0 Td
(been)Tj
-35.6029 -1.2982 Td
(generated)Tj
4.1328 0 Td
(by)Tj
1.1849 0 Td
(signature-tagged)Tj
6.8768 0 Td
(mutagenesis)Tj
5.1534 0 Td
(and)Tj
1.7291 0 Td
(their)Tj
2.1089 0 Td
(insertion)Tj
3.8098 0 Td
(sites)Tj
1.9332 0 Td
(have)Tj
2.0579 0 Td
(been)Tj
2.126 0 Td
(identified)Tj
4.0592 0 Td
([)Tj
0.83 0.64 0.02 0 k
(61)Tj
0 g
(].)Tj
-35.172 -1.2983 Td
(Several)Tj
3.05 0 Td
(mutants)Tj
3.5036 0 Td
(affected)Tj
3.3278 0 Td
(in)Tj
1.0375 0 Td
(nitrogen)Tj
3.6454 0 Td
(fixation)Tj
3.2995 0 Td
(have)Tj
2.0579 0 Td
(been)Tj
2.1203 0 Td
(identified.)Tj
4.286 0 Td
(STM30)Tj
3.1521 0 Td
(has)Tj
1.542 0 Td
(a)Tj
0.652 0 Td
(transposon)Tj
-31.6741 -1.3039 Td
(inserted)Tj
3.4412 0 Td
(in)Tj
1.0318 0 Td
(mll0343,)Tj
3.6623 0 Td
(encoding)Tj
3.9288 0 Td
(glutamine)Tj
4.252 0 Td
(synthetase)Tj
4.3539 0 Td
(I)Tj
0.5556 0 Td
(\(strain)Tj
2.8403 0 Td
(ID)Tj
1.2983 0 Td
(10T05g06\).)Tj
4.7168 0 Td
(The)Tj
1.7802 0 Td
(mutant)Tj
3.1464 0 Td
(is)Tj
0.8504 0 Td
(sig-)Tj
-35.858 -1.2983 Td
(nificantly)Tj
3.9968 0 Td
(reduced)Tj
3.4412 0 Td
(in)Tj
1.0375 0 Td
(its)Tj
1.1452 0 Td
(ability)Tj
2.6929 0 Td
(to)Tj
1.0261 0 Td
(fix)Tj
1.2416 0 Td
(nitrogen,)Tj
3.8777 0 Td
(as)Tj
1.0148 0 Td
(measured)Tj
4.1046 0 Td
(by)Tj
1.1848 0 Td
(acetylene)Tj
3.8948 0 Td
(reduction)Tj
4.1159 0 Td
(\(ca.)Tj
1.6441 0 Td
(45%,)Tj
-34.418 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(62)Tj
0 g
(]\).)Tj
2.415 0 Td
(Glutamine)Tj
4.5014 0 Td
(synthetase)Tj
4.3483 0 Td
(\(along)Tj
2.761 0 Td
(with)Tj
2.0012 0 Td
(glutamine)Tj
4.2463 0 Td
(synthase\))Tj
3.9685 0 Td
(is)Tj
0.8504 0 Td
(responsible)Tj
4.7508 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(assimilation)Tj
-32.7002 -1.3039 Td
(of)Tj
1.0148 0 Td
(ammonium)Tj
4.9605 0 Td
(in)Tj
1.0375 0 Td
(legume)Tj
3.1238 0 Td
(nodules,)Tj
3.5886 0 Td
(which)Tj
2.6532 0 Td
(is)Tj
0.8447 0 Td
(the)Tj
1.4684 0 Td
(main)Tj
2.2904 0 Td
(product)Tj
3.3958 0 Td
(of)Tj
1.0148 0 Td
(nitrogen)Tj
3.6454 0 Td
(fixation)Tj
3.2995 0 Td
([)Tj
0.83 0.64 0.02 0 k
(62)Tj
0 g
(].)Tj
2.0749 0 Td
(The)Tj
-34.4123 -1.2982 Td
(MAFF)Tj
2.8516 0 Td
(mutant)Tj
3.1464 0 Td
(STM6)Tj
2.6759 0 Td
(has)Tj
1.542 0 Td
(a)Tj
0.652 0 Td
(transposon)Tj
4.6771 0 Td
(inserted)Tj
3.4413 0 Td
(in)Tj
1.0318 0 Td
(mlr5906,)Tj
3.787 0 Td
(which)Tj
2.6476 0 Td
(is)Tj
0.8504 0 Td
(the)Tj
1.4683 0 Td
(nitrogenase)Tj
4.8699 0 Td
(gene)Tj
/F8 1 Tf
2.0806 0 Td
[()18()12()]TJ
/F7 1 Tf
-35.7219 -1.2983 Td
(\(strain)Tj
2.8402 0 Td
(ID)Tj
1.2926 0 Td
(17T02d02\).)Tj
4.7792 0 Td
(This)Tj
1.9899 0 Td
(mutant)Tj
3.1465 0 Td
(is)Tj
0.8447 0 Td
(incapable)Tj
4.0251 0 Td
(of)Tj
1.0148 0 Td
(fixing)Tj
2.5172 0 Td
(nitrogen)Tj
3.651 0 Td
([)Tj
0.83 0.64 0.02 0 k
(61)Tj
0 g
(].)Tj
2.0749 0 Td
(Nitrogenases)Tj
5.4198 0 Td
(are)Tj
1.4457 0 Td
(a)Tj
0.6576 0 Td
(fam-)Tj
-35.6992 -1.2983 Td
(ily)Tj
1.1849 0 Td
(of)Tj
1.0147 0 Td
(metalloenzymes)Tj
6.6387 0 Td
(that)Tj
1.7802 0 Td
(catalyze)Tj
3.3505 0 Td
(the)Tj
1.4683 0 Td
(reduction)Tj
4.1159 0 Td
(of)Tj
1.0148 0 Td
(dinitrogen)Tj
4.439 0 Td
(to)Tj
1.0262 0 Td
(ammonia.)Tj
4.2746 0 Td
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1.1112 0 Td
(MAFF,)Tj
3.0784 0 Td
(the)Tj
-34.4974 -1.3039 Td
(majority)Tj
3.6283 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(genes)Tj
2.4434 0 Td
(necessary)Tj
4.0195 0 Td
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1.389 0 Td
(nodulation)Tj
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(and)Tj
1.7291 0 Td
(nitrogen)Tj
3.651 0 Td
(fixation)Tj
3.2939 0 Td
(are)Tj
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(clustered)Tj
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(symbio-)Tj
-34.2309 -1.2983 Td
(sis)Tj
1.2075 0 Td
(island)Tj
2.6022 0 Td
([)Tj
0.83 0.64 0.02 0 k
(59)Tj
0 g
(].)Tj
0.01 Tc
-2.6135 -1.2982 Td
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1.8255 0 Td
(goals)Tj
2.3017 0 Td
(of)Tj
1.0431 0 Td
(our)Tj
1.6724 0 Td
(experiment)Tj
4.9153 0 Td
(were)Tj
2.177 0 Td
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1.0545 0 Td
(\(i\))Tj
1.2075 0 Td
(test)Tj
1.6611 0 Td
(whether)Tj
3.5716 0 Td
(mildly)Tj
2.8687 0 Td
(effective)Tj
3.634 0 Td
(and)Tj
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(ineffective)Tj
-30.903 -1.3039 Td
(mutants)Tj
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/F8 1 Tf
1.0431 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.3436 0 Td
[()4(\n)12()7()]TJ
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1.5421 0 Td
(nodulate)Tj
/F8 1 Tf
3.8437 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
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1.0035 0 Td
[()3()12()4(\n)11()18()4()1()-4( )]TJ
/F7 1 Tf
4.0535 0 Td
(hosts)Tj
2.3414 0 Td
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0.9808 0 Td
(reduced)Tj
3.5433 0 Td
(rates)Tj
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(when)Tj
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(competing)Tj
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(against)Tj
-32.5528 -1.2983 Td
(wildtype)Tj
3.7473 0 Td
(MAFF,)Tj
3.1521 0 Td
(\(ii\))Tj
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(examine)Tj
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(whether)Tj
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(the)Tj
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(investigate)Tj
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(the)Tj
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(sanctions)Tj
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(varying)Tj
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(amounts)Tj
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(nitrogen)Tj
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(fixation.)Tj
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/F8 1 Tf
4.5637 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
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1.0035 0 Td
[()3()12()4(\n)11()18()4()1()-4( )]TJ
/F7 1 Tf
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(hosts)Tj
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(with)Tj
2.058 0 Td
(single)Tj
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(mixed)Tj
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(inocula)Tj
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(STM30,)Tj
3.4639 0 Td
(and)Tj
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(STM6)Tj
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(in)Tj
1.0658 0 Td
(order)Tj
2.4888 0 Td
(to)Tj
1.0545 0 Td
(estimate)Tj
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Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
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q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 392.9952 745.7952 cm
BT
/F6 1 Tf
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-0.005 Tc
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(Lotus)Tj
2.622 0 Td
(japonicus)Tj
/F0 1 Tf
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3.763 0 Td
(fixing)Tj
2.5087 0 Td
(symbion)Tj
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Q
q
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576 47.5002 l
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81.5811 34.9228 m
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h
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BT
/F0 1 Tf
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(ONE)Tj
2.3669 0 Td
(|)Tj
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(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01855)Tj
4.089 0 Td
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2.0906 0 Td
(September)Tj
5.0386 0 Td
(28,)Tj
1.5874 0 Td
(2017)Tj
32.2798 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(15)Tj
ET
Q
q
0.83 0.64 0.02 0 k
1 j
1 J
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38.4378 696.6425 m
101.0268 696.6425 l
h
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0 g
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BT
/F11 1 Tf
1 TL
-0.025 Tc
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4.2661 0 Td
(\(NIFA\))Tj
2.6221 0 Td
(grant)Tj
2.1543 0 Td
(2014-5118)Tj
4.089 0 Td
(1-22384)Tj
-13.1315 -1.3748 Td
(\()Tj
0.83 0.64 0.02 0 k
(https://nif)Tj
3.8409 0 Td
(a.usda.gov/)Tj
0 g
(\).)Tj
5.0386 0 Td
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1.5875 0 Td
(funders)Tj
3.0472 0 Td
(had)Tj
1.5874 0 Td
(no)Tj
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(role)Tj
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-17.8796 -1.3748 Td
(study)Tj
2.2677 0 Td
(design,)Tj
2.8984 0 Td
(data)Tj
1.793 0 Td
(collection)Tj
3.7346 0 Td
(and)Tj
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