· Title
· Authors
· Stage of Progress
· Action items needed
1. Evolutionary erosion of symbiotic function in Bradyrhizobium japonicum
· Sachs, Russell, Hollowell
· Accepted PLoS One 25 Sept, 2011
· Finish
production of figures, final details WILL BE PUBLISHED NOV 2
2. Evolutionary origins and stability of Proteobacterial mutualisms
· Skophammer, Stajich, Bansal, Sachs
· Completion of analysis by Skophammer, Stajich
· Skophammer and Sachs completes writing (Submit fall 2011)
3. Origins and evolution of Type-three Secretion Systems across Proteobacteria
· Skophammer, Fronk, Hollowell, Sachs
· Completion of analysis by Fronk
· Skophammer and Sachs write (Submit fall/winter 2011)
4. ‘Nodule Biogeography’
· Hollowell, Regus, Bernardo, Pham, Sachs
· Completion of sequencing needed (Its,recA, glnII, NodDA, NifD of L. Strigosus nodules from 2005, 2009, 2011 collections // Creation of database with GPS locations needed // haplotype abundance (Its, recA, glnII), symbiosis island haplotypes (NodDA, NifD; null).
· Hollowell, Bernardo, Pham will complete sequencing; Hollowell, Regus will finalize hypotheses to be tested and analyze data. (Submit Spring 2012).
5. ‘Nodule Histology’
· Regus, O’Neill, DeMason, Suzuki, Sachs
· Regus, O’Neill finalize slide preparation and photography
· Regus, O’Neill finalize hypotheses to be tested, analyses, write paper (Submit Spring 2012)
6. Nitrogen supplementation and the maintenance of legume host control (‘Nitrogen addition experiment’)
· Regus, Sachs
· Regus will design experiment // Regus & Gleason will set up inoculation experiments in fall 2011/ Harvest in late 2011
· Regus will set up experiment, analyze data, write paper (Submit mid-2012)
7. Nitrogen supplementation and the emergence of rhizobial parasitism (‘Nitrogen growth curve’)
· Regus, Sachs
· Regus will experiment // Regus & Gleason will set up inoculation experiments in late fall 2011/ Harvest in 2012
· Regus will set up experiment, analyze data, write paper (Submit mid-2012)
8. Host control versus symbiont exploitation in the legume-rhizobium interaction - Review
· Regus, Sachs
· Regus is lead author on review of host control mechanisms in legumes and their effectiveness at contraining rhizobial exploitation
· Regus will write outline // gather meta-data // write paper (Submit Late-2012)
9. Repeated evolutionary loss of symbiosis loci in Bradyrhizobium
· Hollowell, Bernardo, Pham Sachs
· 3 locus phylogenetic analysis must be completed with 2 sym loci mapped on to tree
· Hollowell will complete Phylogeny of all Los2005 strains, perform Nif/Nod/16s blots on all haplotype groups, analyze data, write paper
10. The evolution of resistance and toxicity in wild Bradyrhizobium populations
· Hollowell, Gleason, Sachs
· Hollowell will create hapolotype-grouped phylogeny from 75 inoc strains, will choose separate haplotype strains for analysis, will test all hapolotypes against ~10 diverse antibiotics, will create a matrix of strain interactions to examine toxin production
· Holowell and Gleason will perform assays, Hollowell will analyze data and write paper
11. Host control over Host control over Bradyrhiobium in root biofilm populations
· Bantay, Bernardo, Pham, Sachs
· Bantay, Bernardo and Pham will complete sequencing of LoS from all 2009 isolates (GlnII, RecA, Its, NifD, NodD-A)
· Bantay will analyze data with Sachs, Bantay and Sachs will write paper
12. Phylogenomic analysis of origins and loss of symbiosis in Bradyrhizobium
· Stajich, Bantay, Sachs
· Sachs will isolate genomic DNA, Bantay will vet strains, organize library creation wil Stajich, assemble/analyze sequences
· Stajich and Sachs will assist in data analysis, Sachs and Stajich will write paper